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Neurofibromatosis-1 heterozygosity increases microglia in a spatially and temporally restricted pattern relevant to mouse optic glioma formation and growth

These segments bear the most mechanical stress during daily activities, making them particularly vulnerable to injury and degeneration

Ionotropic glutamate receptors and expression of N-methyl-D-aspartate receptor subunits in subregions of human hippocampus: Effects of schizophrenia
As to the pain "going in" it may be that the needle caught a nerve or vein plus the fact that you were probably a little tense and nervous

10.1016/j.jare.2022.11.003 55 XuX.DaiM.LaoF.ChenF.HuX.LiuY.et al (2020)