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Since lysine must be deprotonated to allow for acetylation, the rate of acetylation is influenced by the protonation state of lysine

The Wernicke-Korsakoff syndrome: A clinical and pathological study of 245 patients, 82 with post-mortem examinations

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Immunostaining was then performed to visualize the cells area using an anti-3 tubulin mouse primary antibody (1:1000) and an anti-mouse secondary antibody coupled with Alexa Fluor 568 (1:1000)
