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Glutathione (GSH) consists of amino acids, namely cysteine, glutamine, and glycine
One key consideration for preparing your glutathione agarose beads is how much volume of beads you need to use for your pull-down
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As appropriate to individual experiments, a phosphatidylinositol 3-kinase (PI3K) inhibitor, LY294002, and/or a nuclear factor-B (NF-B) inhibitor, BAY11-7082, were added 1 h prior to IGF-1 treatment
