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In a healthy system, intracellular glutathione immediately neutralizes these free radicals, protecting the delicate mitochondrial membranes from lipid peroxidation and structural damage
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For immunofluorescence double staining with anti-FASN and anti-8-oxo-dG, paraffin sections were incubated with rabbit anti-FASN (dilution 1:50, Cell Signaling) and mouse anti-anti-8-oxo-dG (1:250, Trevigen) at 4 C o/n in a humidified chamber
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