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The GAPDH gene was served as the internal control ( CT method ( Cloning, expression and identification of rSmGST The first Spirometra GST gene deposited in GeneBank (AEI16476.1) was amplified by PCR with specific primers carrying BamHI and PstI restriction enzyme sites (underlined) (forward, 5-AT GGATCC ATGGGTTC GCTCCCGGTTC-3, and reverse, 5-AT CTGCAG CTAAGCATCACCACGCCAG-3), and the cycling protocol was as follows: 30 cycles of 95C for 50 s, 60C for 50 s and 72C for 50 s

Long-term risks are unknown

The model was conditioned with a medium corresponding to the concentration of metabolites present in human blood (Supplementary Data 11) 60

A total of 98 G6PD-associated genes were identified, and out of the 47 implicated signaling pathways, seven were highly likely to be regulated by G6PD-associated genes

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