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Immediately after washing, the cells were provided with a full cell culture medium and were placed in the incubator of the microscope (37C under a humidified 5% CO 2 atmosphere)

Second, due to the open-label design, both the investigators and participants were aware of the treatment allocation, which could systematically influence the evaluation of therapeutic efficacy

479 Additional signaling pathways, including those involving PKR (EIF2AK2), PERK (EIF2AK3), lysosomal acidification, and sirtuin-regulated antioxidant programs, have also been implicated in shaping the mitochondrial stress response