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Working with cold solutions can interfere with complete dissolution and may affect the peptide's interaction with the diluent

This process ensures that the immune system does not attack its own cells and tissues

The cells were collected after treatment, washed twice with cold phosphate-buffered saline, and treated with 1 mL of cold citrate buffer (0.24 M sucrose, 40 mM sodium citrate, pH 7.6)

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Thats the goal
