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Description
Samples were then precipitated by ice cold H 2 O/MeOH/CHCl 3 (v/v 4:4:1) washed and resuspended in 50 mM HEPES buffer (pH 7.4)

[DOI] [PubMed] [Google Scholar] 242

Diabetes Metabolism J
However, solutions prepared with sterile water lack antimicrobial protection and must be used within 24 hours or stored as single-use aliquots

doi: 10.3390/molecules26092554
