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307 YamadaT.EgashiraN.BandoA.NishimeY.TonogaiY.ImutaM.et al (2012)

C6 cells were transfected at 80% confluence with a combination of GluN1 and GluN2A or GluN2B (3.75 g cDNA/wells) + 10 l of Lipofectamine (Invitrogen) diluted in Opti-MEM TM (Gibco TM )

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Other anti-inflammatory effects of sulforaphane in mice have been based on ultraviolet B (UVB)-induced skin inflammation [225,226], the genetic model of muscular dystrophy [227,228], ischemia/reperfusion-induced muscular injury [229], ischaemia/reperfusion injury and cardiac allograft vasculopathy [127], vascular remodelling in hypoxic pulmonary hypertension [230], aged mice [231], angiotensin II-induced renal inflammation and injury [232], ischaemia/reperfusion injury [233], hypoxia-induced cardiomyopathy [234], the genetic model of kidney disease [235], retinitis Pigmentosa [236], atopic dermatitis [237], UV radiation-induced inflammation [238], acute exhaustive exercise-induced organ damage and inflammation [239], radiation-induced skin damage [240], oxazolone-induced chronic itch model [241], and acute pancreatitis in mouse [158] experimental models