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The V3-V4 hypervariable regions of bacterial 16S rDNA genes were amplified by PCR using specific primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGG GTATCTAAT-3), with the resulting PCR products verified through 2% agarose gel electrophoresis and subsequently purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA)

Genome-wide association study identifies seven novel susceptibility loci for primary open-angle glaucoma

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