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doi: 10.3389/fphar.2020.00718 47 MerlinJIvanovSDumontASergushichevAGallJStunaultMet al

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[DOI] [PMC free article] [PubMed] [Google Scholar] 133.Peng J., Liu Y., Xie J., Yang G., Huang Z

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Purified GST-MFAP2 (full-length) was immobilized on GlutathioneSepharose 4B beads by incubating 20 g of the protein with 30 L of pre-washed beads in binding buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM DTT, and 0.1% Triton X-100) (Beyotime, Shanghai, China) for 1 h at 4 C with gentle rotation