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Methods for Cell-Free Gene Expression Cell-free gene expression (CFE), also referred to as in vitro transcription and translation (IVTT or TX-TL) 21,32 or cell-free protein synthesis (CFPS), 1 is the activation of transcription and translation from cellular components derived from either crude cellular extracts of various organisms 28 (e.g., E

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doi: 10.2174/157340607780620662 31 Rotondo DottoreGChiariniRDe GregorioMLeoMCasiniGCestariLet al

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Results Trapping H 2 S, RSS and RS(S) n S as stable products To quantify H 2 S, RSS and RS(S) n S within samples we developed a sulfur atom trapping approach that incorporates the target S atom into diagnostic products that can be sensitively quantified by LC-MS/MS