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First, the binding of danger associated molecular patterns (DAMPs), pathogen associated molecular pattern (PAMPs), or cytokines to TLRs triggers the activation of NF-B signaling pathway, resulting in an upregulation in the expression of pro-IL-1, pro-IL-18, and NOD-like receptor thermal protein domain associated protein 3 (NLRP3)

Plates were read at 405 nm and titers were defined as the log 3 serum dilution two-fold above background

Organic & biomolecular chemistry 11 , 20982104, (2013)

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Western blotting of lysates from RNAi- gstA2 and gstA2 -OE cells was done by lysis in RIPA (radio immunoprecipitation assay) buffer containing protease inhibitors (Sigma-Aldrich), followed by electrophoresis and immunoblotting with rabbit anti-rDdGSTA2 for 1hour at room temperature (1:2500 dilution)