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The following assays are SET-based methods: Total phenolics assay using FolinCiocalteu reagent Trolox equivalent antioxidant capacity assay (TEAC) Ferric ion reducing antioxidant power assay (FRAP) Total antioxidant potential assay using a Cu 2+ complex as an oxidant 2,2-Diphenyl-1-picrylhydrazyl radical scavenging assay (DPPH) 2,2-Azinobis 3-ethylbenzthiazoline-6-sulfonic acid radical scavenging assay (ABTS + ) N,N-Dimethyl-p-phenylenediamine radical scavenging assay (DMPD + ) Cupric ion (Cu 2+ ) reducing antioxidant power assay (CUPRAC) It has been reported that ABTS methods involve both HAT and SET mechanisms (Prior et al

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Notartomaso S, Mascio G, Bernabucci M, et al

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Assembly of the L-Carnitine Biosynthetic Pathway After efficient enzymes for the last and penultimate step of the L-carnitine biosynthetic pathway had been identified, enzymes for the first two steps were selected: TML hydroxylase and an HTML aldolase (Supplementary Table 1)