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The reaction between RT and GSH is based on a Michael addition reaction that is inherently reversible, has an appropriate dissociation equilibrium constant K d in the mM range and rapid reaction kinetics, and provides ratiometric readouts, thus allowing GSH quantification independent of the probe concentration

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It provides a preserved aqueous vehicle that can be accessed multiple times, making it useful for reconstituting lyophilized research peptides and preparing small-volume stock solutions in laboratory workflows