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Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellmans reagent

The area of any other individual impurity peak should not be greater than 0.2 times the area of the principal peak in the reference solution (0.1%)

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These observations were reaffirmed by Zare and co-workers who used the same doses (0.3 and 0.6 mg/mL) of LC supplementation during in vitro maturation of immature BCB+ (Brilliant Cresyl Blue positive) oocytes