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Tissues were homogenized in 510 mL of cold buffer (50 mM potassium phosphate, pH 7.5, 1 mM EDTA) per gram of tissue, followed by centrifugation at 10,000 g for 15 min at 4 C

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Dead space, wasted peptide, and syringe efficiency Every syringe wastes a small amount of medication

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Through its effects on the metabolism of catecholamine neurotransmitters and estrogen, COMT is emerging as a key player in the pathogenesis of AD

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