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At birth, males homozygous for 5-reductase-2 mutation typically have a clitoral-like phallus, bifid scrotum, hypospadias, blind shallow vaginal pouch from incomplete closure of the urogenital sinus and a rudimentary prostate (Figure 2 and Figure 3)

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Polarized lighting images were performed on a fluorescence microscope (Olympus BX43, Tokyo, Japan) fitted with an analyzer (U-ANT, Olympus) and polarized (U-POT, Olympus)