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The biosynthesis of MSH proceeds from myo-inositol-1-phosphate, UDP-GlcNAc and cysteine and occurs in five steps (Jothivasan and Hamilton, 2008

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Isolation of nuclear and cytoplasmic proteins Nuclear and cytoplasmic proteins were isolated using the NE-PER Nuclear and Cytoplasmic Extraction Reagents kit following the detailed instructions provided by the manufacturer (Thermo scientific, Rockford, IL, USA)

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