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After exposure to a 660 nm laser for different time intervals, the GSH level remaining in the solution was determined using a microreduced GSH assay kit (Solarbio Science & Technology Co., Ltd., Beijing)

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After 24 h, cells were lysed and the luciferase activity was assayed in the lysates using Promegas Dual-luciferase assay system according to the manufacturers protocol (Promega, Madrid, Spain)

reported that GLS-driven Gln metabolism is a regulator of radiotherapy tolerance in prostate cancer (PCa) and that high expression of GLS 1 and c-MYC, key regulators of Gln, are significantly associated with reduced progression-free survival in prostate cancer patients treated with radiotherapy

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