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During the 7-day treatment schedule, MP-13 maintained stable antinociception without tolerance development, as evidenced by the unchanged microglial cell count in the periaqueductal gray, indicating that chronic exposure did not trigger neuroinflammatory adaptationa process in which microglia become activated, release pro-inflammatory cytokines, and contribute to opioid tolerance

Linoleic acid, an essential polyunsaturated fatty acid involved in lipid metabolism and inflammation regulation, was significantly elevated in MG, indicating marked disruption of fatty acid metabolism

Finally, it remains unknown if diet intervention could downregulate plasma L-Cn levels effectively

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