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Description
Decoupling the assay by first starting the transaminase reaction followed by addition of the dehydrogenase did not alter overall velocities concluding that the dehydrogenase reaction must be the rate limiting step in the reaction
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10.1016/j.phrs.2022.106163 97 NyawoT
The extraction solvent consisted of 80% methanol and 20% aqueous solution (pH 7.00) containing 10 mM ammonium formate (Sigma-Aldrich, 70221-25G-F) and 25 mM NEM (Thermo Scientific, 040526.06)
Treatments are gentle and well-tolerated
