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The LC-MS/MS analysis ran for a total of 86.5 minutes following a gradient elution profile: 2% B from 05.5 min, 210% B between 5.5 and 25.5 min, 1025% B from 25.545.5 min, 2537% B over 45.555.5 min, and a ramp to 80% B from 55.585.5 min

doi: 10.1038/s41569-022-00739-0 40
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Response of the vascular system to the administration of the neuropeptide Semax Here, we found changes in the expression levels of several genes involved in the functioning of the vascular system as a response to Semax administration

29 The majority of the nociceptive fibers in the IVD are concentrated in the outer rim of the annulus fibrosis and posterior longitudinal ligament (PLL)
