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Protein samples were separated by 412% SDS-PAGE, transferred onto PVDF membranes (GE Healthcare, Milan, Italy), and incubated with antibodies targeting and Alpha-Smooth Muscle Actin (catalog n A2547, Sigma-Aldrich)

First, peptides have proven clinical utility across multiple therapeutic areas, with particular success in endocrine, oncological, and rare disease applications

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Combining in the syringe doesn't create new compounds or reduce efficacy