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After incubation, and washes with PBS, appropriate secondary fluorescent antibodies (1:500, AlexaFluor-conjugated, Invitrogen) were added to the blocking solution at room temperature for 2 h in the dark
In clinical studies (Yoshino, Brenner) NAD+ precursors (NR, NMN) are administered 250 to 1000 mg/day orally
Individuals with low B12 levels, fatigue, or those following a vegan/vegetarian diet
these proteins can undergo lipoylation and, under conditions of copper excess, abnormally aggregate, triggering cell death (166)
Int J Mol Sci (2014) 15:483755
