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In order to make the animal model of AFLD, all mice except control groups were gavaged a single dose of alcohol each day according to the following method: 10ml (5.14mol/L alcohol) /kg body weight in the first 4 weeks, 11ml (6.85mol/L alcohol) /kg body weight in the second 4 weeks, 12ml (8.56mol/L alcohol) /kg body weight in the final 4 weeks

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Arachidonic acid elicits a substrate-gated proton current associated with the glutamate transporter EAAT4

THD Ascorbate: A more stable alternative to Vitamin C Tetrahexyldecyl Ascorbate (THD Ascorbate) is a more stable, oil-soluble form of Vitamin C

They can be minimized by: Rotating injection sites consistently Using proper injection technique Ensuring the alcohol has dried before injecting Applying ice before injection if sensitive Headaches: Some subjects report mild headaches, particularly during the first week