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For HCT116, 4 NuPAGE LDS sample buffer was added to the cell lysates of (NP0007, Thermo Fisher Scientific) supplemented with 10% DTT and heated at 95 C for 5 min

Specifically, it consists of amino acids 176-191 from the growth hormone molecule, plus an additional tyrosine residue at the N-terminus

N-Acetyl L-cysteine (NAC) is, in contrast, more stable and only loses ~15% of sulphur therefore offers superior antioxidant support, through its promotion of glutathione synthesis