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The column temperature was set to 40 C, and the gradient elution was at 0.35 mL/mL of flow rate: 0 to 3 min, linear gradient from 15% to 70% of Phase A
However, brains of GPx1 deficient mice have increased oxidative stress at baseline, and increased production of ROS in response to kainic acid
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doi: 10.1016/S0731-7085(03)00573-9
Because of the difficulty of obtaining complete urine collections, creatinine clearance calculated in this way is at least as reliable as estimates based on urine collections
