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The absorbance of each well was quantified at 450 nm using an automated ELISA reader (SpectraMax M5, Molecular Devices, United States)
At their pK a value, they exist in equal concentrations and can readily interconvert ions
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However, keeping a clear upgrade path toward a combined Cagrilintide + Semaglutide research kit makes it easier to expand projects later without redesigning the whole supply chain
