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The PI (Sigma, P4170) and Hoechst (Sigma, B2261) mixture was added into the cell culture well and incubated at 37 C for 10 min under dark conditions

One of the significant areas where vitamin B12 shines is its influence on metabolism and body fat

In the context of aging research, NAD+ occupies a position of particular scientific significance: research consistently demonstrates that NAD+ levels decline substantially with age in mammalian tissues, and that this decline correlates with hallmarks of biological aging including mitochondrial dysfunction, impaired DNA repair, and metabolic dysregulation

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