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Swerdlow RH, Burns JM, Khan SM

Through injection of -syn preformed fibrils (PFFs) into the striatum of transgenic mice, researchers demonstrated the development of Lewy pathology, nigrostriatal degeneration, and importantly expanded our understanding of cell-to-cell transmission by describing the nature of spread in neuroanatomically connected regions: this provided the first evidence that synthetic -syn PFFs alone can induce the initiation and propagation of -syn pathology in vivo (Luk et al., The evidence of cell-to-cell transmission of pathologic -syn in interconnected brain regions suggests a prion-like mechanism of spread

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Without direct brain concentration measurements, it remains unclear whether the transcriptional differences observed between treatment paradigms reflect pharmacokinetic differences, exposure duration, or both

Gupta, Gill, Andrews, and Prausnitz, working in human subjects and published in the Journal of Controlled Release in 2011, used electrical impedance to track how quickly skin reseals its barrier after microneedle insertion, and found that in the absence of occlusion, treated sites recovered their barrier properties quickly, on the order of a couple of hours