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It is also the biological rationale for why researchers came to treat the E-domain of the mechanically induced variant as a signal in its own right, distinct from the mature IGF-1 attached to it

Phosphorylated type I receptors recruit and phosphorylate Smad1/5/8 receptors, which bind Smad4 to form complexes that translocate to the nucleus to regulate the expression of multiple downstream target genes

Following 48 h of incubation, the inserts were washed, fixed, and stained with 0.1% crystal violet

At the same time, epithelial cells will migrate and proliferate to cover the wound surface, preventing infection and loss of moisture

Antibiotic selections were performed with ampicillin (Amp) 100 g mL 1 , kanamycine (Km) 50 g mL 1 , or both streptomycin (Sm) 25 g mL 1 and spectinomycin (Sp) 75 g mL 1