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Co-immunoprecipitation In brief, the extracts of SW620 cells were blocked with immunoglobulin G (IgG) or protein A/G agarose (BioWorld, Visalia, USA) for 2 h at 4C to remove nonspecific protein binding, and then incubated with anti-FBX8 (Abcam, Cambridge, UK) or anti-GSTP1 overnight at 4C

RNase H confers specificity in the dnaA-dependent initiation of replication at the unique origin of the Escherichia coli chromosome in vivo and in vitro

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YilmazRStrafellaAPBernardASchulteCvan den HeuvelLSchneiderhan-MarraNet al

If this acute inflammatory phase fails to resolve, persistent immune activation drives a transition to chronic inflammation, characterized by sustained infiltration of monocytes and lymphocytes, neovascularization, and connective tissue remodeling 34,35,36