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Similarly, both in vivo feeding with a choline-rich diet and in vitro trimethylamine-N-oxidase (TMAO) stimulation increased TMAO-dependent NLRP3 inflammasome activation and expression of IL-1, IL6, TNF-, CXCL9, and CXCL10, supporting that NLRP3 is a key proteolytic activator in the macrophage response to a high choline and TMAO production ( Figure 3 )

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BMC Genomics 26:355 Manoil C, Kaiser D (1980) Guanosine pentaphosphate and guanosine tetraphosphate accumulation and induction of Myxococcus Xanthus fruiting body development