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Proteomics Protein extraction Proteins were extracted using RIPA buffer (Fisher Scientific)

The document advocates for increased awareness, targeted campaigns, and proactive care strategies to bridge gaps in the patient care of individuals with diabetes, emphasizing the importance of early detection and timely management to improve overall health outcomes

For the mass spectrometry, a PRM method was employed with an included list for the masses of the metabolites of interest and their optimized normalized collision energies (Supplementary Table 4)