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Once reconstituted, store at 28C and use within the stability window noted on the COA

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The sophisticated process of mitochondrial biogenesis requires coordination between mtDNA and nDNA, including mtDNA transcription and translation, translation of nDNA-encoded transcripts, membrane recruitment, protein import, and assembly of the OXPHOS complexes (Wu et al., 1999), and PPARG coactivator 1 beta (PGC1) along with PPARG related-coactivator 1 (PRC) were discovered subsequently (Lin et al., 2002)

After LPS treatment, the samples were washed with cold PBS and fixed in 4 % paraformaldehyde in PBS for 15 min at room temperature