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The melanocortin-4 receptor (MC4R), a hypothalamic master regulator of energy homeostasis and appetite, is a class A G-protein-coupled receptor and a prime target for the pharmacological treatment of obesity

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Similar to GST pulldowns, nickel agarose (left), streptavidin agarose (middle), and aFLAG agarose (right), are used to detect interactions with his-tagged, strep-tagged, and flag-tagged target proteins, respectively

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