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Oncotarget 8, 7301.CrossRefGoogle ScholarPubMed Oguszka, M, Te Pas, MFW, Poawska, E, Nawrocka, A, Stepanow, K & Pierzchaa, M (2020) Omega-3 Alpha-Linolenic fatty acid affects the level of telomere binding protein TRF1 in porcine skeletal muscle

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doi: 10.1016/j.jcmgh.2020.01.004

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Confocal microscopy For confocal microscopy assay, cells were washed twice with a washing buffer (D-PBS supplemented with 4.5 g/L of glucose and 5 mM of MgCl 2 ), followed by treatment with Cy5-labeled AsiG (250 nM) either free or loaded by liposome with or without anti-CD71 antibody in a binding buffer (D-PBS supplemented with 4.5 g/L of glucose, 5 mM of MgCl 2 , 1 mg/mL BSA, and 0.1 mg/mL of yeast tRNA) for 1 h at pH 7.4 or 6.5 on ice