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The Cyp2e1 antibody was purchased from Proteintech (Rosemont, IL, USA) (Cat

HED/Cys-SG and CHP assays If not otherwise indicated, strep-MBP-GRX samples were reduced with 10 mM DTT for 2 hours and the buffer was exchanged against 100 mM Tris/HCl p H 8.0, 1 mM EDTA 15 using PD MiniTrap G-25 desalting columns (GE Healthcare)

et al., 2021) that emodin mediates autophagy through the AMPK/mTOR signaling pathway, thereby reducing apoptosis and podocyte injury in DKD rats

doi:10.3389/fphys.2021.784852 [29] Hahn J, Cook NR, Alexander EK, et al

A more recent approach utilizes hydroquinone, anilinium sulfate, and ammonium molybdate reactions with unconsumed H 2 O 2 , forming quantifiable purple compounds (Hadwan and Ali, 2018)