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The column temperature was set to 40 C, and the gradient elution was at 0.35 mL/mL of flow rate: 0 to 3 min, linear gradient from 15% to 70% of Phase A

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Here, we outline historical and current thinking regarding the functions of histone modifications and their associated enzymes

At predetermined intervals, 500 L aliquots were collected, and the absorbance at 550 nm was measured using a spectrophotometer to quantify release kinetics