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Pellets were thawed on ice for 1 h and lysed in 200 l 1% SDS in PBS supplemented with 1 protease inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche, 11836153001) and shaken for 30 min at room temperature with 1:1,000 v/v benzonase nuclease (Sigma, E1014)

Stem cell therapy for Parkinsons disease Limitations of current treatments and diagnostic approaches Current PD treatments mainly focus on symptom management, categorized into pharmacological (medications), surgical (e.g., deep brain stimulation for advanced cases), and non-pharmacological approaches (physical therapy, occupational therapy, and lifestyle changes), as seen in Table 3