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Succinate drives the production of IL1, mediated by HIF1 (39) whereas citrate accumulation leads to the production of itaconate, a potent inhibitor of isocitrate lyase, which is necessary for Mtb persistence (43)

Lett 2019, 6, 106111

On the other hand, the NCI H23 cell line, sensitive to cisplatin, shows a mutation at the IVR (intervining-region) domain of KEAP1

Mixtures of the designated purified proteins were incubated with glutathione Sepharose beads or Ni-NTA beads in pull-down buffer (20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM EDTA, 1 mM DTT, 1 protease inhibitor) at 4 C for 2 h