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This work was supported by the generous support of the American people through the United States Agency for International Development (USAID)

Cells were then decalcified with 0.6 mol/L HCl for 24 h

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Neurogenesis is also likely affected by the downregulation of the T-box gene Tbr2/EOMES at this early stage of organoid development, as it is highly expressed in intermediate cortical progenitors in mice (Mihalas and Hevner, 2017) and humans (Cipriani et al., 2016) as part of a transcriptional cascade during glutamatergic neurogenesis

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