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Description
Nat Rev Drug Discov 19(12):884901

The remaining iodoacetamide was quenched by adding 5 mM DTT and the proteins were digested with 200 ng trypsin (Trypsin Gold, Promega) at 37 C ON

Planta 231:609621

Rest assured that there are plenty of solutions out there to resolve B12 deficiency, whether its through sublingual/oral tablets, diet, or injections

Extracted samples were vortexed at 4 C for 10 min, cleared by centrifugation (17,000 g , 10 min, 4 C) and then supernatants were analysed by LCMS
