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Ideally, blood tests (including full blood count, serum B12, folate, and possibly anti-intrinsic factor antibodies if pernicious anaemia is suspected) should be performed before starting supplements, as supplementation can affect test results

Freeze-drying removes moisture so the contents stay shelf-stable as a dry powder during storage and transit until they are prepared in a research setting

Then, the supernatant sera and culture media were filtered through 0.22-m filters and ultracentrifuged at 100,000 g for 12 h at 4 C

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