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BT20, MDA-MB-231, MDA-MB-468, and BT549 cells were purchased from the American Type Culture Collection (ATCC) and cultured in Dulbeccos Modified Eagle Medium (DMEM), except BT549 which was cultured in RPMI, supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin/l-glutamine

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The representative processed results are available at Source data supporting the results in Fig

Although direct evidence of CHAC1 involvement in human neurodegeneration remains limited, its well-established role in GSH catabolism positions it mechanistically at the intersection of ferroptotic pathways