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Schwarzlander et al., 2008

Ent1 and Ent2 show distinctive binding to Chc boxes To comprehend the interaction determinants between Ent1 and Ent2 with Clathrin in vivo we performed acceptor photobleaching FRET experiments between Ent1 or Ent2 endogenously tagged with mNeonGreen and Chc-mScarlet

Flow cytometry For intracellular staining, SC-islets were dissociated, fixed, permeabilized, and stained with primary antibodies for 30 min at RT

We also did not see effects on the complex subunits from the mitochondrial respiratory chain that could be associated with Pyr-sulf protection (Supplementary Fig

These coenzymes can exist in oxidized (NAD + and FAD) or reduced forms (NADH and FADH2)