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Because SLC7A11 exchanges intracellular glutamate with extracellular cystine, the intracellular glutamate concentration decreases, which leads to more Gln uptake and increased glutaminase activity and making these cells dependent on external Gln

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A major breakthrough was achieved in 2005 when Karik and Weissman demonstrated that nucleoside modifications including pseudouridine substitution reduced Toll-like receptor activation and enhanced translation efficiency ( Onpattro , became the first approved RNA therapeutic (siRNA-LNP) ( The COVID-19 pandemic potentiated unprecedented levels of development opportunity, accelerating the first widespread use and validation of mRNA vaccine technology

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