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The development of Doppler ultrasonography has resulted in enormous uses of this technology in various perspectives of animal reproduction practices [27,28,29,30] because it enabled researchers and veterinarians to assess both organ morphology and functions based on vascular perfusion [28, 30]

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The methylation cycle is entirely dependent on a steady supply of specific B-vitamins acting as coenzymes, particularly Vitamin B9 (folate), Vitamin B12 (cobalamin), Vitamin B6, and Vitamin B2 (riboflavin)

However, prolonged L-DOPA use may lead to motor disorders (Tang et al., 2013), but continuous antibiotic use to reduce TMAO can disrupt intestinal ecology, affecting beneficial gut flora, and TMAO levels can reappear a month or more after stopping antibiotics