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After a 1-h incubation at 20 C with agitation worms were washed 4 times in M9 buffer to remove traces of paraquat and spotted on fresh NGM plates with 40 M FUDR and seeded with bacteria

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A nanotechnology-based delivery system like chitosan, carbon nanotubes (CNTs), and polylactic-co-glycolic acid (PLGA) have been studied in vitro and in vivo for the delivery of several therapeutic agents and have shown promising concentrations in the brain after nasal administration [195]